Proteins of the Bacillus stearothermophilus Ribosome
نویسندگان
چکیده
Proteins L30 and 55 from the 50 S and 30 S subunits, respectively, of the Bacillus stearothermophilus ribosome have been crystallized. L30 crystals are tetragonal and the space group is P41212 (or P43212)vwith cell dimensions a = b = 46.3 A and c = 61.4 A. S5 crystals are trigonal with the space group P3,21 (or P3,21) *and cell dimensions a = b = 59.3 A and c = 109.8 A. In both cases, there appears to be a single molecule in the asymmetric unit.
منابع مشابه
Isolation and amino acid composition of ribosomal proteins from Bacillus stearothermophilus.
Twelve of the proteins from the 30S ribosome of Bacillus stearothermophilus were isolated by preparative disc electrophoresis. Amino acid analyses of these proteins showed them to be different from each other. The gross amino acid composition of 30S ribosomal protein from B. stearothermophilus and Escherichia coli are virtually identical. A number of the proteins of B. stearothermophilus had el...
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Ribosome composition, consisting of rRNA and ribosomal proteins, is highly conserved among a broad range of organisms. However, biochemical studies focusing on ribosomal subunit exchangeability between organisms remain limited. In this study, we show that chimeric ribosomes, composed of Escherichia coli and Bacillus subtilis or E. coli and Geobacillus stearothermophilus subunits, are active for...
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The 5 A resolution crystal structure analysis of ribosomal protein L30 from Bacillus stearothermophilus is described. The molecule is shown to be compact and extend to about 25-30 A in each dimension.
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